Summary
Type: SPE
Application #: 301040
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
2 column volumes methanol, then 2column volumes 0.1 M hydrochloric acid
Sample application:
Apply 100 μl plasma to the column (if desired, add 50 μl propazolamide, 4 μg /
ml, as internal standard), let stand 1 min, then force or aspirate sample
through the column, let stand 2 min
Column washing:
1 column volume 0.1 M hydrochloric acid, 1 column volume dist. water, finally
200 μl methanol
Elution:
300 μl methanol
Authors: Macherey-Nagel
Source: LC Department